Purification and identification of small pig bone marrow MSCs

      This study was to carry out a large animal experiments, selection of experimental animals pig with human organs and immune system. Small pigs in Guizhou and Guangxi Bama pigs two inbred miniature pigs for experimental donor, acceptor, the establishment of small the porcine tibial shaft middle 2cm segmental bone defect model. Discussed separation and purification and identification of small pig bone marrow MSCs, allogeneic tissue engineered bone constructed in vitro. According to the characteristics of allogeneic tissue engineered bone graft, using the streaming technology, ELISA, RT-PCR, immunohistochemistry and other methods for monitoring after transplantation rejection, and X-ray examination, organization special staining, biomechanical testing and observation The osteoblasts effect.

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